We narrowed to 14,023 results for: CAN

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pLKO.1-shMBNL3-0455

Plasmid

#115463

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-0455 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRS DV DNM2-mRUBY2

Plasmid

#107793

Purpose

donor vector for knock-in gene editing at human DNM2 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM2-mRuby2 (DNM2 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Expression

Bacterial and Yeast

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-LN

Plasmid

#188231

Purpose

Polycistronic human LIN28 and NANOG were cloned into the pMXs vector

Depositor

Young Tang

Article

Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

Insert

LIN28A and NANOG separated by 2A sequence (LIN28A, NANOG Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-321/+120 mut1&2

Plasmid

#35546

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Mutation

T to G at position -52 and T to G at position -96…

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBS L30 Ruby3-Rab7A

Plasmid

#135651

Purpose

Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Ruby3

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594E

Plasmid

#131714

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594E

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgMARK4

Plasmid

#138691

Purpose

Expresses a human MARK4-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 human (MARK4 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161Y

Plasmid

#98951

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161Y: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161H

Plasmid

#98952

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161H: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAF161

Plasmid

#135926

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

TNFRSF9 fused to mCerulean3

Use

Transposon

Tags

mCerulean3

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean-proα2(I)

Plasmid

#119836

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mCerulean

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd3

Plasmid

#102865

Purpose

Expresses mouse Fzd3 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 3 (Fzd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMiR-E6AP-3'UTR-miR-375-mut

Plasmid

#53695

Purpose

Luciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site on E6AP 3'U…

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pCEP4-myc-CXCR4-H79Q

Plasmid

#98953

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant H79Q

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

H79Q: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

Plasmid

#92225

Purpose

Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP Y35TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Residue Y35 mutated into TAG (Amber) stop codon f…

Promoter

p70a

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p-miR-Report + WT miR-181 BS in CDX2 3UTR

Plasmid

#22792

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pECE-M2-CDC27 S379A

Plasmid

#31662

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 379 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only