305,511 results

1. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p101_CRISPRai_PiggyBac

   Plasmid

   #213777

   Purpose

   Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   VPR-dSaCas9
   dSpCas9-KRAB-BFP
   zeocin
   Tet-On transactivator
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a (EF-1-alpha intron a) and Tet_On_TRE

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p101_CRISPRai_PiggyBac

   Plasmid

   #213777

   Purpose

   Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   VPR-dSaCas9
   dSpCas9-KRAB-BFP
   zeocin
   Tet-On transactivator
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a (EF-1-alpha intron a) and Tet_On_TRE

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.GFAP.eGFP.WPRE.hGH

   Plasmid

   #105549

   Purpose

   AAV expression of EGFP from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV5

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.GFAP.eGFP.WPRE.hGH

   Plasmid

   #105549

   Purpose

   AAV expression of EGFP from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV5

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ER-mScarletI

   Plasmid

   #137805

   Purpose

   Visualization of the Endoplasmic Reticulum

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ER-mScarletI

   Plasmid

   #137805

   Purpose

   Visualization of the Endoplasmic Reticulum

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CamKII-ArchT-GFP (PV2527) (AAV5)

   Viral Prep

   #99039-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition.

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Promoter

   CaMKII

   Tags

   GFP

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

10. mCherry-LaminB1-10

    Plasmid

    #55069

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge