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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Publication

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Publication

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Available since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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PIK3CA - Exon9 E545K

Plasmid

#16642

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

E545K; see note below

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Publication

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Publication

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCEP4-hERG1a

Plasmid

#53052

Purpose

Expresses alpha subunit of hERG1a K channel in mammalian cells

Depositor

Michael Sanguinetti

Publication

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLJM60-FLAG-SLC38A9.1 (delta110)

Plasmid

#71861

Purpose

lentiviral stable expression

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, missing first 110 amino acids

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Publication

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Tags

myc3

Expression

Mammalian

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA-GATA2

Plasmid

#1287

Depositor

Gokhan Hotamisligil

Publication

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Publication

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Tags

His and Myc

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pTRE-GFP-NMHC II-A

Plasmid

#10844

Depositor

Robert Adelstein

Publication

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-A (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Cav3-YFP

Plasmid

#69507

Purpose

Expression of fluorescently tagged Caveolin-3 in mammalian cells

Depositor

Rob Parton

Publication

Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

Insert

Cav3 (Cav3 Mouse)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Publication

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDONR223-RAF1

Plasmid

#23832

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RAF1 (RAF1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pRK5 beta4

Plasmid

#16037

Depositor

Filippo Giancotti

Publication

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Publication

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Publication

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

FLAG

Expression

Mammalian

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBABEpuro-CRAF

Plasmid

#51124

Purpose

mammalian expression of wt CRAF

Depositor

Matthew Meyerson

Insert

CRAF (RAF1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Publication

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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