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pTol2-CreLite

Plasmid

#131783

Purpose

Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC, PIF6CreN, and mTagBFP2

Use

Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

Tags

mTagBFP2

Expression

Mammalian

Promoter

Ubi

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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TCR012H

Plasmid

#233454

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR012H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR012H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPRKCZ-C1-EGFP (C-PRKCZ-C1)

Plasmid

#217759

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Mutation

C1 domain (aa 123-193)

Promoter

CMV

Available Since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 βarr1 S412D Flag

Plasmid

#42196

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412D (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412D

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only
Arrb1-Q394L

Plasmid

#35402

Depositor

Robert Lefkowitz

Article

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Q394L

Promoter

CMV

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

Plasmid

#85179

Purpose

For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) tagRFP

Expression

Mammalian

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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SaBE3-Gam

Plasmid

#100810

Purpose

Expresses SaBE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13

Plasmid

#186223

Purpose

For expression of ATG13 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-407)

Plasmid

#61934

Purpose

Expression of hRPN13 (285-407) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CAGGS-Flex-Turbo/GFP

Plasmid

#197886

Purpose

Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

Turbo/GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMini-CAAGs::RFP-DR48

Plasmid

#132640

Purpose

Fluorescent reporter to quantify elicitation of the strand annealing mediated repair pathway

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

mRFP

Expression

Mammalian

Mutation

Contains a 92bp insertion interrupting the open …

Promoter

Chicken B-actin

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pmKO-AF8

Plasmid

#118224

Purpose

Kusabira- Orange-CENP-A expression plasmid

Depositor

Kenji Sugimoto

Article

Sugimoto et al Mutat Res. 2008 Nov 17;657(1):56-62. doi

Insert

CENP-A (CENPA Human)

Tags

Kusabira orange

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-chCARex

Plasmid

#87233

Purpose

bacterial expression of GST-CAR extracellular domain

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pYSD315

Plasmid

#253034

Purpose

Encodes the Aga2 signal peptide, mRuby2 fluorescent protein, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

mRuby2-649-stalk anchor

Expression

Yeast

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00134_PGK-mNeonGreen-syn_pA-EF1alpha-TagBFP-syn_pA

Plasmid

#252538

Purpose

Constitutive promoters with downstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00135_EF1alpha-TagBFP-syn_pA-PGK-mNeonGreen-syn_pA

Plasmid

#252539

Purpose

Constitutive promoters with upstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40)

Plasmid

#252542

Purpose

Inducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG03426_pLentiX1-EF1alpha-mRuby2-bGH-TRE-TagBFP-bGH

Plasmid

#252550

Purpose

Inducible gene and constitutive reporter with upstream tandem syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Use

Lentiviral

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), TRE3G

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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