We narrowed to 9,669 results for: Coli

1. EcAvs4

   Plasmid

   #188794

   Purpose

   Avs4 from Escherichia coli NCTC11132

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK045p

   Plasmid

   #156335

   Purpose

   Expresses heterologous rRNA

   Depositor

   Ahmed Badran

   Article

   Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

   Insert

   rRNA operon, orthogonal-antiRBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT antiRBS engineered to orthogonal antiRBS, PS e…

   Promoter

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSM019

   Plasmid

   #128291

   Purpose

   asymmetric plasmid partitioning E. coli - regulatory plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Para - lp-sfyfp-parB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. RepX_pET28a(+)

   Plasmid

   #172199

   Purpose

   Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

   Depositor

   Taekjip Ha

   Article

   Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

   Insert

   RepX superhelicase
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   C18L, C43S, C167V, S400C, C612A

   Promoter

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDA303

   Plasmid

   #195199

   Purpose

   Overexpressing 6His-TetR in E. coli.

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

   Insert

   TetR
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas1+2

   Plasmid

   #72676

   Purpose

   Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

   Depositor

   Udi Qimron

   Article

   Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

   Insert

   Cas1, Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMA2775

   Plasmid

   #46884

   Purpose

   Lentiviral vector encoding Tet-inducible mitochondrially targeted myc-tagged E.coli exonuclease III

   Depositor

   Mikhail Alexeyev

   Article

   Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

   Insert

   exonucleaseIII E. coli
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-Tight

   Available Since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-Ec.coaA

   Plasmid

   #50386

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Erick Strauss

   Article

   Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

   Insert

   PanK
   

   Use

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMUT2-pFF-41.5-mCherry

    Plasmid

    #192863

    Purpose

    pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Oxygen inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMUT2-pCadBA-mCherry

    Plasmid

    #192865

    Purpose

    pMUT2 based vector for sensing pH in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    pH sensitive promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLRD16

    Plasmid

    #208268

    Purpose

    Production of syn-stemar-13-ene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    osCPS4
    osKSL8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLRD13

    Plasmid

    #208265

    Purpose

    Production of ent-cassa-12,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    ptmT2
    osKSL7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLRD05

    Plasmid

    #208259

    Purpose

    Production of isopimara-8,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    cldB
    sare_1287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMUT2-pLac-mCherry

    Plasmid

    #192859

    Purpose

    pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    lldR operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET24c(+)-EFTs

    Plasmid

    #31173

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Ts
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

20. Ec2Avs2

    Plasmid

    #188790

    Purpose

    Avs2 from Escherichia coli NCTC10650

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Ec2Avs2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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