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pDEST17-hOPTN

Plasmid

#23053

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

optineurin (OPTN Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFMRPiso1I304N

Plasmid

#48692

Purpose

Expresses hFMRP iso 1 I304N

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 I304N (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mTurquoise2

Plasmid

#215539

Purpose

Expression vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mTurquoise2

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFRT-DestFLAGHA_RBPMS

Plasmid

#65743

Purpose

constitutively expresses RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pDEST-ERBB2-v2

Plasmid

#154895

Purpose

Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pVTU-Dest

Plasmid

#105088

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pGGDestSC+ATG

Plasmid

#49323

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDest-303

Plasmid

#159678

Purpose

Gateway destination vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2D4

Plasmid

#15786

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D4 (UBE2D4 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-cDAXX

Plasmid

#169729

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

6 His

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST_5xUAS:EB3-mScarlet-I

Plasmid

#192358

Purpose

EB3 tagged with mScarlet-I, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

EB3 (MAPRE3 Human)

Tags

mScarlet-I

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_HuB

Plasmid

#65758

Purpose

constitutively expresses FLAG-HA-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestGFP_HuB

Plasmid

#65761

Purpose

expresses GFP-tagged HuB in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL2 (ELAVL2 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST15 hSlp4-a

Plasmid

#40046

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
Puro-iDEST

Plasmid

#75336

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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