Addgene

We narrowed to 2,166 results for: ada.2

Showing: 1101 - 1120 of 2166 results

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pCMV4a-SIRT2-Flag

Plasmid

#102623

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

Sirtuin 2 (SIRT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)

Plasmid

#185822

Purpose

A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

Plasmid

#140658

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT) mAID-EGFP-Nluc TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(WT)

Plasmid

#140729

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone2

Plasmid

#162120

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone3

Plasmid

#162121

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(F74G)-V5

Plasmid

#158664

Purpose

Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(F74G) AAVS1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

F74G

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ERT2-iCre-ERT2

Plasmid

#178059

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human synapsin 1 (Syn) promoter

Available since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-ERT2-iCre-ERT2

Plasmid

#178060

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human ubiquitin C (UBC) promoter

Available since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Tags

Expression

Mutation

See depositor comments below.

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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RAD21 C-tag CRISPR

Plasmid

#140540

Purpose

RAD21 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEGPN-TMPRSS2

Plasmid

#187484

Purpose

Expression vector - TMPRSS2

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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