We narrowed to 7,357 results for: ef1a

1. pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA

   Plasmid

   #37756

   Purpose

   Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-TdTomato
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   TdTomato

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1A-CD19.8H.28TM.BB.28.3z

   Plasmid

   #200677

   Purpose

   Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-DIO-mCherry-WPRE

   Plasmid

   #202620

   Purpose

   Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

   Plasmid

   #185060

   Purpose

   Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lv EF1a HP1cs-Frb2x PGK Puro

   Plasmid

   #102809

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   HP1cs-Frb2x
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1553 - pAAV EF1a EGFP-KASH

   Plasmid

   #131682

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Insert

   EGFP-KASH
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-MinD-IRES-Puro

   Plasmid

   #213114

   Purpose

   Expression of untagged MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA

   Plasmid

   #37086

   Purpose

   Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-YFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid

    #135682

    Purpose

    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-DIO-GRAB_g5-HT2m

    Plasmid

    #208722

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHage2-EF1a-mCherryTRF1-IRES-Blast

    Plasmid

    #182588

    Purpose

    expresses mcherry-trf1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

    Plasmid

    #160507

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

    Plasmid

    #69587

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-Coff/Fon-sRGECO

    Plasmid

    #137128

    Purpose

    Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTYF-EF1a-DLDH-IRES-EGFP

    Plasmid

    #178310

    Purpose

    Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    D-lactate dehydrogenase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1a-AcGFP-P2A-hBRD4S

    Plasmid

    #197882

    Purpose

    study roles of human BET proteins in human pluripotency reprogramming

    Depositor

    Kejin Hu

    Article

    Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1A-LivePAR(R163A)-Hygro

    Plasmid

    #187609

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

    Depositor

    Robert Sobol

    Article

    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

    Insert

    LivePAR(R163A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R163A mutation in the WWE Domain

    Promoter

    EF1A

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-CCre-MBP4-WPRE

    Plasmid

    #193919

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP4
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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