We narrowed to 5,282 results for: pcdna3.1

1. pcDNA3.1(+)_Nav1.6(K1546TAG)-HA

   Plasmid

   #239764

   Purpose

   Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K1546TAG

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_Nav1.6(WT, Y371C)-HA

   Plasmid

   #239765

   Purpose

   Expresses TTX-resistant (Y371C) variant of mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Y371C

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GSDMD_1-270

   Plasmid

   #218229

   Purpose

   Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 1-270

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA3.1-GSDMD_1-275

   Plasmid

   #218230

   Purpose

   Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 1-275

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNA3.1-GSDMD_29-484

   Plasmid

   #218231

   Purpose

   Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 29-484

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA3.1-GSDMD_29-270

   Plasmid

   #218232

   Purpose

   Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 29-270

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pcDNA3.1-HaloAKAR2.1-CLC

   Plasmid

   #216492

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1-CLC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-flag-IARS2-Δ1-48

   Plasmid

   #232341

   Purpose

   Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-48 (indicated as Mito-signal in UniPro…

   Promoter

   CMV

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-FLAG-Rgs14

   Plasmid

   #236396

   Purpose

   Expresses FLAG-Rgs14 in mammalian cells

   Depositor

   John Hepler

   Article

   Shu et al Cell Signal. 2007 Jan;19(1):163-76. doi:

   Insert

   Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1+ dgat1b-FLAG

    Plasmid

    #232144

    Purpose

    Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1b
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Flag-Sintbad

    Plasmid

    #234668

    Purpose

    expresses SINTBAD (TBKBP1) in mammalian cells

    Depositor

    Lienhard Schmitz

    Article

    Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #234754

    Purpose

    Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-hSyncytin-1(AAAR)

    Plasmid

    #182442

    Purpose

    hSyncytin-1, furin cleavage site RNKR>AAAR(314-317)

    Depositor

    Masaharu Somiya

    Insert

    ERVW-1 (ERVW-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    furin cleacage site RNKR>>AAAR(314-317)

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-hSyncytin-1(deltaFP)

    Plasmid

    #182443

    Purpose

    hSyncytin-1, fusion peptide deletion(320-340)

    Depositor

    Masaharu Somiya

    Insert

    ERVW-1 (ERVW-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    no fusion peptide (320-340)

    Promoter

    CMV-F

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-hSyncytin-1(C186A)

    Plasmid

    #182450

    Purpose

    hSyncytin-1, fusion deficit and dominant negative mutant (C186A)

    Depositor

    Masaharu Somiya

    Insert

    ERVW-1 (ERVW-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C186A

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Flag-TUBG2

    Plasmid

    #229855

    Purpose

    TUBG2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Insert

    TUBG2 (TUBG2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Removal of the internal TUBG2-EcoRI site by chang…

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 N-T2A-M-IRES-E

    Plasmid

    #231903

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203M in N protein

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 FFSS-BACH1

    Plasmid

    #232273

    Purpose

    Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1+ dgat1a-FLAG

    Plasmid

    #232143

    Purpose

    Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1a (dgat1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1+ mogat3b-FLAG

    Plasmid

    #232145

    Purpose

    Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3b
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge