We narrowed to 13,992 results for: GAN

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lentiCRISPR v2-sgPlscr3-2#

Plasmid

#234772

Purpose

knockout Plscr3

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Plscr3 (Plscr3 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgPlscr3-1#

Plasmid

#234767

Purpose

knockout Plscr3

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Plscr3 (Plscr3 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R82A_pET-14b

Plasmid

#233276

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 82…

Promoter

T7 promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R64A_pET-14b

Plasmid

#233263

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 64…

Promoter

T7 promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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HemH-R212A_pET-14b

Plasmid

#233262

Purpose

Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

HemH (hemH )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 21…

Promoter

T7 promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#1-puro

Plasmid

#231984

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

N-terminal His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

Plasmid

#213522

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed V290 to A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

Plasmid

#213523

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed N306 to K

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-EREG-ScNeo

Plasmid

#209915

Purpose

To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-EREG-ScNeo (EREG, TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.BMI1

Plasmid

#224711

Purpose

Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

BMI1 (BMI1 Human)

Tags

6x histidine tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD80-ST3-His

Plasmid

#210665

Purpose

Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCI AscI MR1 res

Plasmid

#214752

Purpose

expresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cells

Depositor

David Lewinsohn

Article

Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

Insert

MHC class I-related protein 1 (MR1 Human)

Tags

IRES eGFP

Expression

Mammalian

Mutation

silent mutations to prevent editing by CRISPR/Cas…

Promoter

CMV

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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