We narrowed to 171,760 results for: addgene

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pET28-MHL: nsp16_SARS-CoV-2

Plasmid

#167850

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1a-IRES-Puro PFKM

Plasmid

#201638

Purpose

Over-expression of untagged human PFKM

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcg 2.0-BFP-circ-arRNA backbone

Plasmid

#213965

Purpose

pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMKO shRNA Bim

Plasmid

#17235

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bim shRNA (BCL2L11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
non-targeting control gRNA (BRDN0001145598)

Plasmid

#80173

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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TNIK gRNA (BRDN0001146045)

Plasmid

#75849

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNIK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TNIK (TNIK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-Ce67

Plasmid

#124686

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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LiPOP1-NanoLuc

Plasmid

#168265

Purpose

Chemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)

Depositor

Yubin Zhou

Article

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Insert

MLKL(1-125) (MLKL Human)

Tags

mCherry, NanoLuc, CRY2-PHR

Expression

Mammalian

Mutation

H15A, K16A, R17A

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1N-LZ-LOV2(fast)

Plasmid

#107690

Purpose

N-terminal half of π-EB1 with fast LOV2, no fluorescent tag

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

A. sativa phototropin 1 LOV2 domain (I427V, H519L…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Promoter

CMV

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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XLone-BSD Cas13d P2A mCherry

Plasmid

#155183

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

CasRx

Use

Unspecified; Piggybac transposon

Tags

NLS and P2A mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAdTrack-CMV HA ALAS-1

Plasmid

#22746

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

ALAS-1 (Alas1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCKC775

Plasmid

#226626

Purpose

piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
EF1a promoter, sfGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

7xTCF-LEF and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC764

Plasmid

#226625

Purpose

piggyBAC pCAG-hCas9-T2A-Unbiased3

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, S. pyogenes, Human)
EF1a promoter, blasticidin resistance

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC776

Plasmid

#226627

Purpose

piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
EF1a promoter, mCherry

Use

Synthetic Biology

Tags

oxygen dependent degron (ODD)

Expression

Mammalian

Promoter

4xHRE_YB TATA and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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