We narrowed to 168,735 results for: addgene

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mEos3.2FP

Plasmid

#203757

Purpose

Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

NRas c-term (NRAS Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-SRF

Plasmid

#170704

Purpose

doxycycline-inducible overexpression of SRF

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SRF (SRF Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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eA3A-BE3 (pRZ206)

Plasmid

#131315

Purpose

CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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RPS6KA2 gRNA (BRDN0001147334)

Plasmid

#75732

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA2 (RPS6KA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK24_HUMAN_D0

Plasmid

#79742

Purpose

This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK24 (STK24 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSICO-CPSF6-358-eGFP

Plasmid

#110693

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-eGFP (CPSF6 )

Use

Lentiviral

Tags

EGFP

Mutation

Truncation of CPSF6 isoform 2 at amino acid 358

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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PKN3 gRNA (BRDN0001146586)

Plasmid

#77947

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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hA3A-BE3 (pRZ215)

Plasmid

#131314

Purpose

CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001147733)

Plasmid

#75576

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001162525)

Plasmid

#75578

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIKFYVE gRNA (BRDN0001147303)

Plasmid

#76892

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV Gpat4-V5

Plasmid

#175145

Purpose

Lentiviral expression of mouse Gpat4-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Gpat4 (Gpat4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PKN1 gRNA (BRDN0001162558)

Plasmid

#76278

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRB112

Plasmid

#180173

Purpose

pAAV construct GluN1WT (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

WT

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 DEPTOR

Plasmid

#21337

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 1 (Deptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-HK2_sgRNA1

Plasmid

#201596

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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PHKG1 gRNA (BRDN0001144981)

Plasmid

#76356

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKG1 (PHKG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-NONO

Plasmid

#46325

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

NONO (NONO Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
PGK1 gRNA (BRDN0001144730)

Plasmid

#77980

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_Cre

Plasmid

#201198

Purpose

AAV vector for cre expression under EF1 promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

cre
EF1alpha promoter

Use

AAV

Expression

Bacterial and Mammalian

Mutation

nuclear targeting cre

Promoter

N/A and hEF1alpha promoter

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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