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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,304 results for: crispr

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Showing: 11221 - 11240 of 25304 results

  5. ScFv-2ERT2-VPH

    Plasmid
    #120556
    Purpose
    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1
    Depositor
    Yu Wang
    Publication
    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25
    Insert
    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hPGK
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    120556-plasmid-map-sequence-id-252529

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  6. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Tom Ellis
    Publication
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Expression
    Bacterial
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109391-plasmid-map-sequence-id-210784

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  7. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  9. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

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  13. dSp-Rta 125-175

    Plasmid
    #99668
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (125-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99668-plasmid-map-sequence-id-189832

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  15. dSp-p65 (1-100)

    Plasmid
    #99660
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (1-100)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99660-plasmid-map-sequence-id-189821

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  16. dSp-p65 (50-150)

    Plasmid
    #99661
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (50-150)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99661-plasmid-map-sequence-id-189830

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  18. dSp-Rta 75-190

    Plasmid
    #99663
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (75-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99663-plasmid-map-sequence-id-192026

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  19. dSp-Rta 75-175

    Plasmid
    #99666
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (75-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99666-plasmid-map-sequence-id-192042

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Showing: 11221 - 11240 of 25304 results