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pHH0103_PACSIN2-1/1

Plasmid

#91330

Purpose

Protein expression and purification of human SH3 domain construct PACSIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN2-1/1 (PACSIN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pY013 (pcDNA3.1-hEeCpf1)

Plasmid

#69985

Purpose

Expresses humanized EeCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hEeCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-DIO-mCherry-WPRE

Plasmid

#202620

Purpose

Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-5/5

Plasmid

#91464

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-5/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-5/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-1/5

Plasmid

#91351

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-1/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-3/5

Plasmid

#91352

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-3/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Tags

FLAG

Expression

Yeast

Promoter

GAL10

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Flag3-pG-mNanog-pgk-hph

Plasmid

#74911

Purpose

Mammalian expression of Flag tagged mNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4-p100SS/AA (SS866/870AA)

Plasmid

#27555

Depositor

Shao-Cong Sun

Article

Xiao et al EMBO J. 2001 Dec 3. 20(23):6805-15.

Insert

p100 (NFKB2 Human)

Expression

Mammalian

Mutation

Serines 866 and 870 mutated to Alanines

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pX330-HA-dSpCas9

Plasmid

#92112

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9

Use

CRISPR

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtMIR390a-NbSu-2

Plasmid

#213400

Purpose

Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

Depositor

Alberto Carbonell

Article

Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

Insert

Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene

Expression

Plant

Promoter

2x35S

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX9-1/1

Plasmid

#91513

Purpose

Protein expression and purification of human SH3 domain construct SNX9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX9-1/1 (SNX9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-JEDI-1P-WPRE

Plasmid

#202616

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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p-mCherry-N1-iChloC

Plasmid

#85467

Purpose

improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Insert

iChloC

Tags

mCherry

Expression

Mammalian

Mutation

E83Q, E90R, E101S, T159C, D156N

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pY019 (pcDNA3.1-hPmCpf1)

Plasmid

#69991

Purpose

Expresses humanized PmCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPmCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pY009 (pcDNA3.1-hSsCpf1)

Plasmid

#69981

Purpose

Expresses humanized SsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hSsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_RUSC1-1/1

Plasmid

#91379

Purpose

Protein expression and purification of human SH3 domain construct RUSC1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RUSC1-1/1 (RUSC1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2803

Plasmid

#63646

Purpose

pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 mito roGFP2-Orp1

Plasmid

#65001

Purpose

Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_SLC2A2

Plasmid

#45267

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SLC2A2 (SLC2A2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only