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Showing: 11261 - 11280 of 66082 results

  1. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid
    #182532
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Publication
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    hM4Di-mCherry
    Expression
    Mammalian
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182532-plasmid-map-sequence-id-361681

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  4. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde)

    Viral prep
    #162381-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  8. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Publication
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

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  9. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  11. pKAR2-Br512g3.1

    Plasmid
    #171200
    Purpose
    Expresses Br512g3.1 polymerase in E.coli
    Depositor
    Andrew Ellington
    Publication
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171200-plasmid-map-sequence-id-339557

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  18. pM2s2AsG

    Plasmid
    #137924
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Publication
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137924-plasmid-map-sequence-id-331367

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Showing: 11261 - 11280 of 66082 results