168,290 results

1. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC9A7

   Plasmid

   #132187

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A7 (SLC9A7 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS158

   Plasmid

   #215679

   Purpose

   Empty repair plasmid for ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRUPATH Triple Gai3

   Plasmid

   #196050

   Purpose

   Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai3-RLuc8 (GNAI3 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pP2A-mCherry-N1

   Plasmid

   #84329

   Purpose

   Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   P2A-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2061

   Plasmid

   #141900

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMG-AIS

   Plasmid

   #225133

   Purpose

   For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. TFORF0591

    Plasmid

    #142654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO4 (FOXO4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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