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We narrowed to 14,060 results for: crispr grnas

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Showing: 11281 - 11300 of 14060 results

  3. DBJS-p2.13

    Plasmid
    #246892
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for G3BP2 Nterm.
    Depositor
    David Bartel
    Insert
    G3BP2 Nterm Guide RNA 1 (G3BP2 Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246892-plasmid-map-sequence-id-490577

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  4. pX459-puro-hCASP8

    Plasmid
    #185378
    Purpose
    For mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)
    Depositor
    Ayaz Najafov
    Insert
    CASP8 (CASP8 Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185378-plasmid-map-sequence-id-363829

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  5. pX459-puro-hARAF-1

    Plasmid
    #185367
    Purpose
    For mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185367-plasmid-map-sequence-id-363617

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  6. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

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  7. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  8. B-HeFSpCas9

    Plasmid
    #126766
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126766-plasmid-map-sequence-id-259607

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  9. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  10. B-evoSpCas9

    Plasmid
    #126765
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126765-plasmid-map-sequence-id-410921

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  11. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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  12. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  13. pAAV-CMV-Dpi2Cas9-puro

    Plasmid
    #232113
    Purpose
    Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232113-plasmid-map-sequence-id-462529

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  14. pAAV-CMV-Slu1Cas9-puro

    Plasmid
    #232111
    Purpose
    Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232111-plasmid-map-sequence-id-462528

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  15. pAAV-CMV-Slu2Cas9-puro

    Plasmid
    #232112
    Purpose
    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232112-plasmid-map-sequence-id-462527

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  16. pAAV-CMV-SeqCas9-puro

    Plasmid
    #232110
    Purpose
    Expresses SeqCas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232110-plasmid-map-sequence-id-462526

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  17. pENTR_Wheat_live_Cas9

    Plasmid
    #126880
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126880-plasmid-map-sequence-id-249730

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  18. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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Showing: 11281 - 11300 of 14060 results