We narrowed to 13,693 results for: crispr cas9

1. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

   Plasmid

   #123614

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A)-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. BE3(E63Q)-P2A-EGFP (pRZ189)

   Plasmid

   #123613

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(E63Q)-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   E63Q in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LHZ1493

   Plasmid

   #222102

   Purpose

   Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPSG779

   Plasmid

   #239532

   Purpose

   CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae

   Depositor

   John Dueber

   Insert

   sgRNA_YBR197C
   

   Use

   CRISPR

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SeqCBE

   Plasmid

   #232115

   Purpose

   Expresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genes

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SeqABE

   Plasmid

   #232114

   Purpose

   Expresses ABEmax, nSeqCas9 (D10A), and puromycin resistance genes

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE2-SpRY

   Plasmid

   #159979

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTopo_Ex2Donor_pPGK-PURO

   Plasmid

   #198862

   Purpose

   Donor template for CRISPR/Cas9 targeting of TERT Ex2

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   PURO
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 7b sgRNA for EJ7-GFP reporter

    Plasmid

    #113624

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7b sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

11. 7i sgRNA for 4-μHOM reporter

    Plasmid

    #113626

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7i sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

12. 7h sgRNA for 4-μHOM reporter

    Plasmid

    #113625

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7h sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-PE2-NG

    Plasmid

    #159977

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-NG
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE2-VRQR

    Plasmid

    #159983

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VRQR
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE2-VQR

    Plasmid

    #159982

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VQR
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK281 (mCherry2-Hygro)

    Plasmid

    #72797

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTJV1Sc-rpoB

    Plasmid

    #166978

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    Ec86 Retron (ECD_00831 )
    lambda bet
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101 and VanCC

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE2-VRER

    Plasmid

    #159984

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VRER
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK278 (mClover-Hygro)

    Plasmid

    #72794

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Hygro
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK279 (mClover-Bsr)

    Plasmid

    #72795

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Bsr
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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