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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,362 results for: lat

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Showing: 11321 - 11340 of 36362 results

  1. pCDNA3-EMTB-TurboID-V5

    Plasmid
    #190736
    Purpose
    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Publication
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ensconsin (MAP7 Human)
    Tags
    Myc, TurboID, and V5
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190736-plasmid-map-sequence-id-379320

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  9. AICSDP-83:EZH2-mEGFP

    Plasmid
    #164499
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164499-plasmid-map-sequence-id-324903

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  10. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  11. pRC/CMV-TYK2V678F-VSV

    Plasmid
    #139349
    Purpose
    Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)
    Depositor
    Sandra Pellegrini
    Publication
    Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    Tags
    VSV
    Expression
    Mammalian
    Mutation
    V362F-V678F
    Promoter
    CMV
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    139349-plasmid-map-sequence-id-275735

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  12. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid
    #135015
    Purpose
    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus
    Depositor
    David Sibley
    Publication
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    Use
    Cryptosporidium expression
    Tags
    mCherry and Nluc-neo
    Promoter
    Cryptosporidium actin and enolase
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135015-plasmid-map-sequence-id-266476

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  13. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  15. p-mCherry-C1-Aurora_CA

    Plasmid
    #98170
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct Aurora_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    CMV (+enhancer)
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    98170-plasmid-map-sequence-id-182711

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  16. pGLS3-5xHRE-p53(K120R/K164R)

    Plasmid
    #72556
    Purpose
    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.
    Depositor
    Ester Hammond
    Publication
    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p53 (TP53 Human)
    Tags
    5XHRE
    Expression
    Mammalian
    Mutation
    K120R/K164R
    Promoter
    E1B minimal promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    72556-plasmid-map-sequence-id-173934

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  18. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid
    #59826
    Purpose
    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Separase (ESPL1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59826-plasmid-map-sequence-id-99124

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Showing: 11321 - 11340 of 36362 results