336,021 results

1. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

   Viral Prep

   #137141-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Flp-dependent)

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pAAV.CamKII(1.3).eYFP.WPRE.hGH (AAV1)

   Viral Prep

   #105622-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CamKII(1.3).eYFP.WPRE.hGH (#105622). In addition to the viral particles, you will also receive purified pAAV.CamKII(1.3).eYFP.WPRE.hGH plasmid DNA. Expression of EYFP from CaMKII(1.3) promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII(1.3)

   Tags

   EYFP

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pAAV.CamKII(1.3).eYFP.WPRE.hGH (AAV1)

   Viral Prep

   #105622-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CamKII(1.3).eYFP.WPRE.hGH (#105622). In addition to the viral particles, you will also receive purified pAAV.CamKII(1.3).eYFP.WPRE.hGH plasmid DNA. Expression of EYFP from CaMKII(1.3) promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII(1.3)

   Tags

   EYFP

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA Flag Lamp2b-HA

   Plasmid

   #71292

   Purpose

   Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   Flag, HA, and Lamp2 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA Flag Lamp2b-HA

   Plasmid

   #71292

   Purpose

   Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   Flag, HA, and Lamp2 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP1E3D1

    Plasmid

    #20066

    Purpose

    Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

    Insert

    MSP1E3D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeats of …

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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