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pSADdeltaG-ChR2-mCherry

Plasmid

#32646

Purpose

Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

ChR2

Use

Rabies virus

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CTS-PC1

Plasmid

#41576

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7 and myc/myc/his

Expression

Mammalian

Mutation

FLS (4184-4303)

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD-HaloGFP-Na2.4

Plasmid

#228470

Purpose

Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Bacterial

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP18-tetR_O

Plasmid

#127021

Purpose

Basic Part O- ORF; TetR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part TetR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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myr-palm_2xmEYFP

Plasmid

#127811

Purpose

Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mEYFP-mEYFP

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGImC_Empty

Plasmid

#92015

Purpose

Empty backbone, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PE_26: Para_Lac_Tet_eG::sfYFP

Plasmid

#104887

Purpose

PE_26: Para_Lac_Tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Para_Lac_Tet_eG

Expression

Bacterial

Promoter

Para_Lac_Tet_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_TMv2_Zeo

Plasmid

#131762

Purpose

frameshift reporter for barcode retrieval (with Zeo selection)

Depositor

Paul Blainey

Article

Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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hBax S184E C3-EGFP

Plasmid

#19743

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184E (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 184 to Glutamate

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
GP777

Plasmid

#164533

Purpose

Expresses Smo noK-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7025

Plasmid

#128810

Purpose

Fluoride riboswitch-regulated catechol 2,3-dioxygenase

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated catechol 2,3-dioxygenase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pNRB150

Plasmid

#36451

Depositor

Douglas Bishop

Article

Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

Insert

DMC1 (DMC1 Budding Yeast)

Tags

6His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Tags

6His-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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CasRX_Pac

Plasmid

#194803

Purpose

CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

Depositor

Alexander Paredez

Article

Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

Insert

CasRX

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-p110α-WT

Plasmid

#55709

Purpose

eukaryotic expression of Flag tagged p110a WT

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 SAT

Plasmid

#128804

Purpose

overexpression of DDX21 SAT mutation in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 SAT mutation

Tags

flag

Expression

Mammalian

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-dCas9

Plasmid

#190129

Purpose

Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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