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Showing: 1121 - 1140 of 2771 results

  2. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid
    #223106
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223106-plasmid-map-sequence-id-447626

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  3. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid
    #223107
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223107-plasmid-map-sequence-id-447623

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  4. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid
    #223108
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223108-plasmid-map-sequence-id-447631

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  5. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid
    #223109
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223109-plasmid-map-sequence-id-447628

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  7. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

    Plasmid
    #216664
    Purpose
    Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    ETV2 (Etv2 Mouse)
    BFP
    HygromycinR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    216664-plasmid-map-sequence-id-432192

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  13. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and TRE3G
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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  16. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219688-plasmid-map-sequence-id-435723

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  17. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219686
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219686-plasmid-map-sequence-id-436048

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  18. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218426
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27
    Insert
    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of both…
    Promoter
    CMV
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218426-plasmid-map-sequence-id-434963

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  19. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

    Plasmid
    #218425
    Purpose
    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A
    Depositor
    Dieter Willbold
    Article
    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27
    Insert
    GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    5X: K2A, K13A, R15A, K20A, K23A mutations of down…
    Promoter
    CMV
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218425-plasmid-map-sequence-id-434958

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Showing: 1121 - 1140 of 2771 results