We narrowed to 13,582 results for: RING

1. pCMV5D HA-PPM1M_H flap

   Plasmid

   #236719

   Purpose

   Expresses PPM1M with PPM1H flap domain sequence

   Depositor

   Suzanne Pfeffer

   Article

   Claire Y. et al bioRxiv, 2025

   Insert

   PPM1M_H flap (PPM1M, PPM1H Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PPM1M flap domain replaced with PPM1H flap domain

   Promoter

   CMV

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry

   Plasmid

   #225590

   Purpose

   AAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2 (S54L+T127A)-T2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T-3-TRC8 513-664

   Plasmid

   #185352

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   TRC8 (RNF139 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 513-664 only

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

   Plasmid

   #225588

   Purpose

   Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2(S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

   Plasmid

   #225584

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2 (S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T21R-F8.2 (S54L+T127A)

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-PIAL2M-sim1

   Plasmid

   #67908

   Purpose

   Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

   Depositor

   Andreas Bachmair

   Article

   Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

   Insert

   PIAL2 (AT5G41580 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   E281-A496; VEDL 425-428 AAA

   Promoter

   P-lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRosettav2

   Plasmid

   #115557

   Purpose

   To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments

   Depositor

   John Doench

   Insert

   Hygromycin-Blasticidin-Puromycin-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAH031

   Plasmid

   #90330

   Purpose

   Promoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.

   Depositor

   David Savage

   Insert

   iLOV
   

   Use

   Tags

   None

   Expression

   Mutation

   Promoter

   None

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATSR

   Plasmid

   #50052

   Purpose

   expresses t.gondii VWA+TSR1 domains in mammalian cells

   Depositor

   Timothy Springer

   Insert

   MIC2-ATSR
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   S158A

   Promoter

   Chick beta-actin promoter

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. proATSR

    Plasmid

    #50053

    Purpose

    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-proATSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A

    Promoter

    chick beta-actin promoter

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

11. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. A5TSR

    Plasmid

    #50358

    Purpose

    expresses T.gondii MIC2(67-593) domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-A5TSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A, N357S, N463S, N470D

    Promoter

    CHICK beta-actin promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET-C

    Plasmid

    #213496

    Purpose

    Encodes Node C of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    lambda cI 5G6GP
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. A6TSR

    Plasmid

    #50359

    Purpose

    expresses T.gondii MIC2(67-660) domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-A6TSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A, N357S, N463S, N470D

    Promoter

    CHICK beta-actin promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. MIC2

    Plasmid

    #50050

    Purpose

    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2
    

    Use

    Tags

    HIS, SBP, and TEV

    Expression

    Mammalian

    Mutation

    S158A, N357S, N463S, N470D

    Promoter

    CMV

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCC1R

    Plasmid

    #213497

    Purpose

    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. m2ap

    Plasmid

    #50051

    Purpose

    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells

    Depositor

    Timothy Springer

    Insert

    M2AP
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    starts at aa47; N127R, S158A, S177N, N357S, N463S…

    Promoter

    CMV

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pHelper_NoMutL_V2_gentR

    Plasmid

    #205293

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHelper_Ec1_V1_gentR

    Plasmid

    #205291

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHelper_Ec1_V1_ampR

    Plasmid

    #205292

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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