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pLIB-GST-TEV-RSP3(160-516)

Plasmid

#176692

Purpose

Expression of Chlamydomonas reinhardtii RSP3 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP3

Tags

GST

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-HISX6-TEV-RSP4

Plasmid

#176693

Purpose

Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP4

Tags

HISX6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP5

Plasmid

#176694

Purpose

Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP5 (RSP5 Chlamydomonas reinhardtii)

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP6

Plasmid

#176695

Purpose

Expression of Chlamydomonas reinhardtii RSP6 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP7

Plasmid

#176696

Purpose

Expression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP7

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP9

Plasmid

#176697

Purpose

Expression of Chlamydomonas reinhardtii RSP9 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP9

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP10

Plasmid

#176698

Purpose

Expression of Chlamydomonas reinhardtii RSP10recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP10

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP11

Plasmid

#176699

Purpose

Expression of Chlamydomonas reinhardtii RSP11 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP11

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP12

Plasmid

#176700

Purpose

Expression of Chlamydomonas reinhardtii RSP12 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP12

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP16

Plasmid

#176701

Purpose

Expression of Chlamydomonas reinhardtii RSP16 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP16

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP23

Plasmid

#176702

Purpose

Expression of Chlamydomonas reinhardtii RSP23 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP23

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pBR9-genomic_RSP6-HAX3

Plasmid

#176703

Purpose

Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Tags

HAX3

Expression

Plant

Mutation

None

Promoter

rbcs2

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-H2B-mGFP

Plasmid

#173892

Purpose

for H2B labeling

Depositor

Ron Vale

Article

Yan et al J Cell Biol. 2021 Feb 1;220(2). pii: 211

Insert

H2B (H2BC21 Human)

Use

Lentiviral

Tags

mGFP

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-GFP

Plasmid

#126653

Purpose

Inducible expression of Draper-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Availability

Academic Institutions and Nonprofits only

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pMT Crk-mCherry

Plasmid

#126659

Purpose

Inducible expression of Crk-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT vCrk-GFP

Plasmid

#126661

Purpose

Inducible expression of vCrk truncation in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Expression

Insect

Mutation

residues 1-182

Availability

Academic Institutions and Nonprofits only

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pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT FRB-Draper INT-GFP

Plasmid

#126665

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain

Expression

Insect

Mutation

K986N- see depositor comments below

Availability

Academic Institutions and Nonprofits only

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pMT FRB-Draper INT-mCherry

Plasmid

#126666

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain mCherry

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge