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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,253 results for: mKate

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Showing: 1121 - 1140 of 1253 results

  1. pOCC204

    Plasmid
    #118894
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mKate2, HIS6,…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118894-plasmid-map-sequence-id-232313

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  4. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid
    #157984
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    157984-plasmid-map-sequence-id-355759

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  5. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid
    #157985
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157985-plasmid-map-sequence-id-332449

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  6. pMTB-Multibow-mfR

    Plasmid
    #60991
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKate2
    Use
    Zebrafish
    Tags
    membrane tag
    Promoter
    Bactin2
    Available Since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. CRIMPkit

    Plasmid Kit
    #1000000225
    Purpose
    Universal and highly efficient CRISPR/Cas9 targeted insertional mutagenesis system for zebrafish.
    Depositor
    Robert Bryson-Richardson
    Application
    Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only

  13. pMTB-Multibow-hfR

    Plasmid
    #60998
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKate2
    Use
    Zebrafish
    Tags
    histone 2B tag
    Promoter
    Bactin2
    Available Since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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  15. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

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  16. px330_P53 exon 7 gRNA

    Plasmid
    #68351
    Purpose
    px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 7 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68351-plasmid-map-sequence-id-119623

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Showing: 1121 - 1140 of 1253 results