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pTYB3-human PUM1-HD MUT3-2 (C935S-Q939E)-intein-CBD

Plasmid

#73302

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Use

Tags

intein

Expression

Bacterial

Mutation

C935S-Q939E

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pRRG203-notum

Plasmid

#99067

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea notum

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

notum in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS4B

Plasmid

#178846

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)

Plasmid

#41888

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai C CI cat-/EE

Use

Tags

Expression

Bacterial

Mutation

changed amin acids: 77,78,431 and 432 to glutamate

Promoter

t7

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pRS303 Bt δCOP

Plasmid

#73871

Purpose

Bos taurus Arcn1

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 Promoter- COPD

Use

Tags

Expression

Yeast

Mutation

Promoter

Ret2 Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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274_pSKB3-Venus-LUMP

Plasmid

#65976

Purpose

Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

Venus and thrombin site (11-residue linker that h…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Use

Tags

6xHis-TEV

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AG

Plasmid

#182155

Purpose

Expression of chimeric AG (N-mApple-intron-C-eGFP)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AG

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GA

Plasmid

#182156

Purpose

Expression of chimeric GA (N-eGFP-intron-C-mApple)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GA

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-AA

Plasmid

#182157

Purpose

Knockin plasmid with homologous arm for making zMADM-AA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-GG

Plasmid

#182158

Purpose

Knockin plasmid with homologous arm for making zMADM-GG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-AG

Plasmid

#182159

Purpose

Knockin plasmid with homologous arm for making zMADM-AG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-GA

Plasmid

#182160

Purpose

Knockin plasmid with homologous arm for making zMADM-GA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmKate1-N1

Plasmid

#31906

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

alpha-actinin LSS-mKate1

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pET:GCAT:GFP

Plasmid

#60736

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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[GFP][IRES][Bla]

Plasmid

#97194

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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