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pLenti CRISPR V2 sgMTFMT_1

Plasmid

#106318

Purpose

Express Cas9 and sgRNA targeting MTFMT

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTFMT

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 WT

Plasmid

#111807

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (gig Fly)

Tags

3xFlag

Expression

Insect

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago2K533AK570A

Plasmid

#14104

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Lys570 to Alanine

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Ptn in situ probe

Plasmid

#45632

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Ptn in situ probe (Ptn Mouse)

Use

In situ

Mutation

fragment contains bp# 1241-1830 of NM_008973

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
hSTARR-seq_SCP1 vector_blocking 3

Plasmid

#99318

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9-HF

Plasmid

#192142

Purpose

Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9-HF

Use

AAV

Expression

Mammalian

Mutation

Sha2Cas9-HF (R247A and N415A)

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpeCas9

Plasmid

#192139

Purpose

Expresses SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197965

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-nCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-Sesn1b

Plasmid

#61867

Purpose

Transient overexpression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pEY68

Plasmid

#191065

Purpose

ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-14 (ceh-14 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB2802

Plasmid

#63655

Purpose

pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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PB-BE3-2NLS

Plasmid

#119031

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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p6611 MSCV-IP N-HAonly UBOX5

Plasmid

#35010

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

UBOX5 (Ubox5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pRS315e_pGal-dCas9-SH3

Plasmid

#79613

Purpose

Expresses dCas9-SH3 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

SH3 domain

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Lyn-Y-GECO1

Plasmid

#58200

Purpose

Express the membrane targeted Y-GECO1 in neurons

Depositor

Robert Campbell

Article

Zhao et al Integr Biol (Camb). 2014 May 19.

Insert

Y-GECO1.0

Use

AAV

Promoter

hSyn

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR2C2_Hormone-recep

Plasmid

#109837

Purpose

Protein expression and purification of NR2C2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR2C2_Hormone-recep (NR2C2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP(K10)

Plasmid

#121908

Purpose

Expresses BirA and eCPX-AP(K10)

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 10 in the AP to alanine

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab8b

Plasmid

#109658

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab8b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab8b (rab8b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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