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pOPINK-OTUD6B (OTU, aa 133-293)

Plasmid

#61419

Purpose

Expresses human OTUD6B (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-132.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS1-1/3

Plasmid

#91547

Purpose

Protein expression and purification of human SH3 domain construct SORBS1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS1-1/3 (SORBS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS1-3/3

Plasmid

#91553

Purpose

Protein expression and purification of human SH3 domain construct SORBS1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS1-3/3 (SORBS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS1-2/3

Plasmid

#91530

Purpose

Protein expression and purification of human SH3 domain construct SORBS1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS1-2/3 (SORBS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Ntn3-Fc-His

Plasmid

#72105

Purpose

Expresses the entire Netrin 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn3 (Ntn3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP G116A

Plasmid

#123098

Purpose

Expresses 3xFLAG-GABARAP G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(L)-AP-His

Plasmid

#72025

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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NIPBL D8.4 gRNA

Plasmid

#90803

Purpose

3rd generation lentiviral gRNA plasmid targeting human NIPBL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NIPBL (Guide Designation D8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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NIPBL D7.4 gRNA

Plasmid

#90802

Purpose

3rd generation lentiviral gRNA plasmid targeting human NIPBL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NIPBL (Guide Designation D7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-Fc-His

Plasmid

#72142

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
UBE2C H5.2 gRNA

Plasmid

#90934

Purpose

3rd generation lentiviral gRNA plasmid targeting human UBE2C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UBE2C (Guide Designation H5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB1-1

Plasmid

#121533

Purpose

sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB1-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLV V5-nurim

Plasmid

#175110

Purpose

Lentiviral expression of V5-tagged mouse Nrm

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Nrm (Nrm Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Sema4b-AP-His

Plasmid

#72029

Purpose

Expresses the extracellular region of the Sema4B protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4b (Sema4b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGfa28-nLac

Plasmid

#53130

Purpose

Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.

Depositor

Michael Brenner

Article

Lee et al Glia. 2006 May;53(7):677-87.

Insert

Gfa2 (GFAP Human)

Use

Mouse Targeting

Tags

LacZ, NLS, and mP1

Expression

Mammalian

Mutation

contains the A and B regions (bp -1757 to -1489) …

Promoter

human Gfa2

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-H302A

Plasmid

#115618

Purpose

Expresses human Parkin H302A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

H302A

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)

Plasmid

#135440

Purpose

Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Sema4g-AP-His

Plasmid

#72034

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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