We narrowed to 17,150 results for: tes

Showing: 11401 - 11420 of 17150 results

Results per page:

Sort results by:

p21-HA(RxL)-NeoR

Plasmid

#215106

Purpose

Expresses p21-HA(RxL) in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Tags

HA

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Tet-On-ARNT-shRNA1

Plasmid

#166910

Purpose

Lentivirus for inducible knockdown of human ARNT

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNT shRNA (ARNT Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Tet-On-ARNT-shRNA2

Plasmid

#166911

Purpose

Lentivirus for inducible knockdown of human ARNT

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNT shRNA (ARNT Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Tet-On-ARNT-shRNA3

Plasmid

#166912

Purpose

Lentivirus for inducible knockdown of human ARNT

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNT shRNA (ARNT Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

Plasmid

#208554

Purpose

Lentiviral vector expressing gRNA targeting human BCL11B-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B-E1(14) (BCL11B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV-EF1a-mCherryCXCR4-W

Plasmid

#200102

Purpose

Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR and Lentiviral

Mutation

silent mutations (c.180C>T, 438G>A, 495C>…

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CRISPR-Puro-sgRPL3-56

Plasmid

#208979

Purpose

pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

Depositor

Quanfu Ma

Insert

sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6 in tandem

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS-kkv-R896K::NG

Plasmid

#138957

Purpose

Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

Depositor

Paul Adler

Article

Adler et al bioRxiv 2020

Insert

kkv-R896K::NG (kkv Fly)

Tags

Neon Green fused to C terminus of Kkv

Expression

Insect

Mutation

R896K

Promoter

UAS

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

Plasmid

#196896

Purpose

Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

N-Cadherin-2xHA (Cdh1 Mouse)

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196085

Purpose

Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Myc-PSK2 (K57A)

Plasmid

#197118

Purpose

Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (K57A) (TAOK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 1+linker2

Plasmid

#176918

Purpose

express Granulin 1 with linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1+linker2 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPHR-mCherry-MECP2

Plasmid

#181888

Purpose

Soluble light-dependent effector protein (Methyl-CpG binding protein) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-MECP2 (Mecp2 Mustard Weed, Mouse)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-α-COPI-WD40-Asp115Ala

Plasmid

#180760

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Asp115Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Asp115Ala, Leu…

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 4+linker5

Plasmid

#176924

Purpose

express Granulin 4 with linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4+linker5 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CMVtight-PLXNB2 iOE

Plasmid

#176849

Purpose

lentiviral vector for doxycycline-inducible PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

full length PLXNB2

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-flag-RAP1B-V12-puro

Plasmid

#156168

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-RP-CoV2/RBD-puro

Plasmid

#161793

Purpose

Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 RBD

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge