168,311 results

1. AAVS1 EGFP-LC3B HRD

   Plasmid

   #207551

   Purpose

   Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-LC3B
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1 EGFP-GABARAPL1 HRD

   Plasmid

   #207552

   Purpose

   Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-GABARAPL1
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS77

   Plasmid

   #215681

   Purpose

   Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_MmaPylT_EF1_NES-MmaPylRS WT

   Plasmid

   #174901

   Purpose

   expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG and NES

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP24_T24_pRham-ilvA-relE

   Plasmid

   #201531

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE
   

   Use

   Synthetic Biology

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-SEMA3E-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156970

   Purpose

   Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3E (SEMA3E Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL05006A1C09-pc2

   Plasmid

   #26538

   Depositor

   Phillip Newmark

   Article

   Collins et al PLoS Biol. 2010 . 8(10):e1000509.

   Insert

   prohormone convertase 2
   

   Available Since

   Nov. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

8. MSCV-Luciferase-IRES-NrasG12D

   Plasmid

   #60834

   Purpose

   Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   NrasG12D (Nras Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TB204

   Bacterial Strain

   #230033

   Purpose

   Fluorescently labelled (monomeric sfGFP) E. coli

   Depositor

   Tobias Bergmiller, Calin Guet

   Article

   Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

   Bacterial Resistance

   None

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

10. PLIN2-V5-APEX2

    Plasmid

    #170573

    Purpose

    A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    PLIN2 (Plin2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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