We narrowed to 37,174 results for: INO

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pNOC-ARS-CRISPR-v2

Plasmid

#99863

Purpose

Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

Plasmid

#176573

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTM1_VP35_ZEBOV

Plasmid

#68121

Purpose

Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP35 ZEBOV (VP35 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pBTK527a

Plasmid

#110606

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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REM1

Plasmid

#55681

Purpose

expression of mouse REM1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM1 (Rem1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pNL(CMV)TK2ALuc2/CMV/WPREdU3

Plasmid

#163965

Purpose

Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

HSV-1 TK and FLuc

Use

Lentiviral

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTM1_VP30_ZEBOV

Plasmid

#69119

Purpose

Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP30 ZEBOV (VP30 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTECH-MmAcK3RS(IPYE)

Plasmid

#104071

Purpose

Expression plasmid encoding MmAcK3RS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MmAcK3RS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

Plasmid

#186227

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

mRNA synthesis of rapalog-induced cell adhesion molecule

Tags

V5

Expression

Mammalian

Mutation

FRB (T2098L)

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal TDP43

Plasmid

#27466

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

Plasmid

#159583

Purpose

Luciferase reporter for ribosome pausing

Depositor

Shintaro Iwasaki

Article

Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

Inserts

XBP1u pause site
EIF2S3 5UTR

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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Nme2sgRNA_pLKO

Plasmid

#119926

Purpose

Nme2Cas9 U6-driven sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

NmeCas9 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110δ

Plasmid

#34893

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110δ (PIK3CD Human)

Use

Adaptor plasmid

Mutation

See depositor comments below.

Available Since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMAH-EcaYRS

Plasmid

#153558

Purpose

Amber suppression for incorporating 3-aminotyrosine to proteins in mammalian cells

Depositor

Huiwang Ai

Article

Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

Inserts

EcaYRS
amber suppression tRNA (multiple copies)

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBLADE_ONLY_C

Plasmid

#168050

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Expression

Bacterial

Promoter

J23101**

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-LAMP1-mRFP

Plasmid

#169004

Purpose

Express rat LAMP1-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-mRFP

Use

Unspecified

Tags

mRFP

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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