We narrowed to 18,394 results for: URE

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pFastBac-GST-DAXX-His

Plasmid

#175781

Purpose

DAXX protein for baculovirus expression system

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

GST and TEV

Expression

Insect

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-HygTK

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 FS1

Plasmid

#113955

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, one base pair dele…

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-Spo20(PABD)

Plasmid

#221758

Purpose

Stable expression of GFP-Spo20(PABD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Spo20 (SOS1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem Spo20(PABD) (amino acids 422-551)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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A*02:02:01:01

Plasmid

#203277

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:02:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiPELO #2

Plasmid

#229020

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-ΔD/E-His

Plasmid

#179618

Purpose

Express GST-DAXX-ΔDE-His in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 424-495

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-WT-T373A-S608A-S612A-puro

Plasmid

#212674

Purpose

Express tagged hRb mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

T373A/S608A/S612A

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 puro

Plasmid

#72567

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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A*02:11:01

Plasmid

#203280

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:11:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

Plasmid

#117005

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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eNS2B47NS3 L30SF31S

Plasmid

#155319

Purpose

DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor

Tags

His tag

Expression

Bacterial

Mutation

L30 to S, F31 to S

Promoter

M13

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_USP7_MATH

Plasmid

#178490

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

USP7_MATH (USP7 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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5XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111545

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChrimson_tdtomato_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-egfp-U3-UL8

Plasmid

#166694

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)

Use

Lentiviral

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-D/E-His

Plasmid

#179619

Purpose

Express DAXX D/E region in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

GST, His, and TEV

Expression

Bacterial

Mutation

amino acids 414-505 of DAXX

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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A*66:01:01

Plasmid

#203291

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*66:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_CTTN-1/1

Plasmid

#91550

Purpose

Protein expression and purification of human SH3 domain construct CTTN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CTTN-1/1 (CTTN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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