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pLenti_CMV: Flag-Antares_SV40:PuroR

Plasmid

#183049

Purpose

Expresses FLAG-tagged Antares in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

Antares BRET reporter

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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FL-mCherry

Plasmid

#78862

Purpose

DULIP negative control (prey) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S

Plasmid

#108128

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-BE1

Plasmid

#73019

Purpose

Expresses BE1 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_KD

Plasmid

#79011

Purpose

For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Mutation

K47R

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

R175H

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-jGCaMP8m

Plasmid

#213010

Purpose

To study calcium signaling in Astrocytes

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-jGCaMP8m

Use

AAV

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUD-MSP1D1

Plasmid

#161608

Purpose

Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

UD-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans and tyrosines w…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRGEB32-BAR

Plasmid

#126072

Purpose

For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

Depositor

Charles Hunter

Article

Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSIM24-GUS

Plasmid

#67882

Purpose

Plant gene expression vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

uidA

Expression

Plant

Mutation

T120A

Promoter

M24

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTrc-GWS1B

Plasmid

#239582

Purpose

High copy vector for expression of GWS1B Rubisco

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

GWS1B Rubisco (6X his)

Tags

6X histag

Expression

Bacterial

Mutation

Wild-type

Promoter

Trc

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl-ENT N-1F (R1103A)

Plasmid

#216548

Purpose

For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Tags

Flag

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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Sg-A

Plasmid

#83807

Purpose

Express sgRNA targeting SA site

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb720-HO

Plasmid

#141201

Purpose

Expresses mRhubarb720 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb720
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, L196Q, T202D, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDA083

Plasmid

#131389

Purpose

eGFP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

eGFP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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CAG sHRPa-NRX

Plasmid

#73143

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Tags

2x HA tag and Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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