We narrowed to 15,824 results for: LEA
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Plasmid#140111PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmVenusExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
mApple-hCOL1A1
Plasmid#140113PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmAppleExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb
Plasmid#182009PurposeCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell linesDepositorInsertIgKchL_HA_STf_myc_PDGFRtmb
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES EGFP
Plasmid#136620PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS52-V5-6His
Plasmid#175096PurposeV5 tagged VPS52 is expressed in mammalian cellsDepositorInsertVPS52 (VPS52 Human)
UseTagsV5ExpressionMammalianMutationN666D- please see depositor commentPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2B-Electra1
Plasmid#179478PurposeNuclear labeling with blue fluorescent protein Electra1 fused to H2BDepositorInsertH2B-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
cr3Vn
Plasmid#184861Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLBac-FluB-trimer
Plasmid#229859PurposeInfluenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)DepositorInsertsPA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)
UseTags2x-Streptag and 8xHis-tagExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP mCherry-ApoB-EGFP
Plasmid#112859PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFPDepositorInsertmCherry-apoB-EGFP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucNanobody IRES EGFP
Plasmid#136619PurposePlasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-EnhancerNb-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRRL_U2AF1_S34Y_mCherry
Plasmid#84018Purposeexpression of mutant U2AF1 in mammalian cellsDepositorInsertU2AF1 (U2AF1 Human)
UseLentiviralTagsFLAG and T2A-mCherryExpressionMammalianMutationS34YPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
nls-Flamindo2
Plasmid#73939PurposeExpresses biosensor for cAMP in mammalian cells with nuclear localization signalDepositorInsertnls-Flamindo2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseTagsExpressionMammalianMutationQ76V and F77WPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046899
Plasmid#145608PurposeBacterial Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
UseTagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only