253,803 results

1. pGEX6P1-mCherry-Nanobody

   Plasmid

   #70696

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   mCherry-Nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5_mCherry_P2AT2A_GFP-nb-KS

   Plasmid

   #238233

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-KS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCVneo-HA-ER-Hoxb8

   Plasmid

   #222291

   Purpose

   The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

   Depositor

   Mark Kamps, David Sykes

   Article

   Wang et al Nat Methods. 2006 Apr;3(4):287-93.

   Insert

   ER-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The estrogen receptor hormone binding domain cont…

   Promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1)

   Viral Prep

   #172921-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (#172921). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor jGCaMP8s in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Promoter

   Syn

   Tags

   mRuby3

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

7. alpha7nACHR_Nacho_GCAMP7s

   Plasmid

   #182940

   Purpose

   alpha7 nACHR expression in mammalian cell lines with calcium reporter

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-U6

   Plasmid

   #120358

   Purpose

   piggyBac transposon vector for sgRNA expression.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-U6

   Plasmid

   #120358

   Purpose

   piggyBac transposon vector for sgRNA expression.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_PCP-T5Exo

    Plasmid

    #214736

    Purpose

    encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_PCP-T5Exo_bGH
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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