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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pME18S-F-R-hb2AR

Plasmid

#149367

Purpose

Expresses human b2AR in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

b2AR (ADRB2 Human)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-1/3

Plasmid

#91472

Purpose

Protein expression and purification of human SH3 domain construct NCK1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-1/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NLS

Plasmid

#124625

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCIneo-EloC

Plasmid

#31982

Depositor

Joan Conaway

Article

Brower et al J Biol Chem. 1999 May 7;274(19):13629-36

Insert

Elongin C (ELOC Human)

Expression

Mammalian

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-CuSeCat

Plasmid

#42568

Depositor

Ivan Korendovych

Article

Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

Insert

mutated calmodulin gene

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Valin…

Promoter

T7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mut mod

Plasmid

#48165

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Mut (Mut Mouse)

Use

Luciferase

Mutation

A/T mutation in the ATG of the uORF

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfPENK-2.2k-Venus-WPRE

Plasmid

#156400

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAT9993-Hypa-ABE

Plasmid

#163001

Purpose

plasmid expressing Hypa-ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:SPRED1

Plasmid

#118851

Purpose

Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

SPRED1 (SPRED1 Zebrafish, Human)

Use

Zebrafish expression, tol7

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUHD 10.3-human MIZ1 delta POZ

Plasmid

#74160

Purpose

Tet inducible expression of MIZ1 delta POZ

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 delta POZ (ZBTB17 Human)

Expression

Mammalian

Mutation

delta POZ domain (first 109 aa)

Promoter

CMV2

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only
pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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ERAS

Plasmid

#55656

Purpose

expression of mouse ERAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

ERAS (Eras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
GEM

Plasmid

#55680

Purpose

expression of mouse GEM

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

GEM (Gem Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_SHANK1-1/1

Plasmid

#91517

Purpose

Protein expression and purification of human SH3 domain construct SHANK1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK1-1/1 (SHANK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEL065

Plasmid

#124896

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NGv1)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC

Plasmid

#100031

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC

Tags

1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

Expression

Mammalian

Promoter

Adenovirus major late promoter

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47451

Purpose

AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE BB(N136_0.5NG_C63)-SID4X

Use

AAV; Tale

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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