We narrowed to 51,543 results for: des.1
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Plasmid#45223DepositorInsertGFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)
TagsTEV S15MutationE172GAvailable SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-sp-flag-hSema4D
Plasmid#190656PurposeExpresses Human Sema4D with N terminal flag after signal peptideDepositorAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-sp-myc-mSema4D
Plasmid#190645PurposeExpresses Mouse Sema4D with N-terminal myc after endogenous Signal peptideDepositorAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_1-3
Plasmid#210629PurposeLuciferase experiment for TAL1 promoter 1-3DepositorAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mMib1-FLAG
Plasmid#37116DepositorInsertMib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
Tags3 x FLAGExpressionMammalianMutationACC Kozak sequence added before ATG annotated.PromoterCMVAvailable SinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_B
Plasmid#74375PurposegRNA_B to knockout human AMPK alpha 1 using Cas9nDepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTargetF-FRT
Plasmid#113637PurposeDerivative of pTargetF with gRNA targeting FRT siteDepositorInsertsgRNA-FRT
UseCRISPRPromoterpJ23119Available SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-T2A-GFP-PGK-Puro
Plasmid#203607PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFPDepositorInsertNKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
UseHuman targetingAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_A
Plasmid#74374PurposegRNA_A to knockout human AMPK alpha 1 using Cas9nDepositorAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-p38 β/MAPK11 5'-UTR
Plasmid#115357Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNADepositorAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ins:GCaMP6s; cryaa:RFP
Plasmid#110285PurposeA zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.DepositorInsertGCaMP6s
UseZebrafishPromoterinsulinAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-Ce67
Plasmid#124686PurposeTuning expression of PD-1DepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delSS
Plasmid#177615PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseExpressionMammalianMutationSlippery sequence deletedAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT3000
Plasmid#122536PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_SNAP_NR1
Plasmid#170458PurposeExpresses SNAP tagged NR1 in neuronal cellsDepositorAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
aCat DeltaTS (delta570 to 670 aa in aCat TS WT)
Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
ExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-tTA
Plasmid#127091PurposeAn AAV genome with Cre-dependent expression of tTA from the CAG promoterDepositorInserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterCAGAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR55
Plasmid#106026PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)DepositorInsertOpto-GPR55 (GPR55 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only