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pAC-EsaR-I70V/D91G

Plasmid

#47644

Purpose

pAC-EsaR with a208g and a272g mutations in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V/D91G

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine and Aspartic acid…

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pJTB122

Plasmid

#248913

Purpose

expresses yeast Cak1 in bacteria

Depositor

Edward Winter

Article

Rimal et al J Biol Chem. 2022 Oct;298(10):102450. do

Insert

CAK1 (CAK1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut

Plasmid

#223368

Purpose

Expression of ECOR31 Hachiman mutant (HamA E138A, K140A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamA E138A, K140A)

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pJMP2656

Plasmid

#248701

Purpose

Z. mobilis CRISPRi version 2, promoter C, empty guide

Depositor

Jason Peters

Article

Eckmann et al mSystems. 2025 Dec 4:e0100125. doi: 10.1

Insert

empty sgRNA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCRISPRa-Theo-1

Plasmid

#240207

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSpRYa-Theo-1

Plasmid

#240208

Purpose

Bacterial SpRYa vector expressing dSpRY, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dSpRY
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPRa-Trp

Plasmid

#240209

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a tryptophan-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Tryptophan MR-scRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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DoS1Δinc

Plasmid

#244464

Purpose

DoS1 derived plasmid without RP4 inc

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GG

Plasmid

#235119

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SlmiR482bTS-B/c

Plasmid

#234368

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK883

Plasmid

#242919

Purpose

Constitutive expression of dCas9, MCP-SoxS-SYNZIP6, and αNTD-SYNZIP5

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS-SYNZIP6

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCK071

Plasmid

#242907

Purpose

Constitutive expression of dCas9 and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK157

Plasmid

#242908

Purpose

Constitutive expression of dCas9, MCP-SYNZIP6, and αNTD-SYNZIP5

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Inserts

MCP-SYNZIP6
αNTD-SYNZIP5

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK147.02

Plasmid

#242909

Purpose

Constitutive expression of dCas9, PspF-λN22, and boxB scRNA J102

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA boxB J102

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK692

Plasmid

#242910

Purpose

Constitutive expression of dCas9 and αNTD-MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

αNTD-MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK321

Plasmid

#242912

Purpose

aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR3-SV40-puro

Plasmid

#240537

Purpose

Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR3

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR2-SV40-puro

Plasmid

#240536

Purpose

Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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DoS2-pCU1

Plasmid

#244470

Purpose

Targeting pCU1 plasmid

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

I-SceI enzyme (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

tetR/tetA promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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