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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 11501 - 11520 of 14359 results

  4. 53BP1 N-terminal sgRNA

    Plasmid
    #207091
    Purpose
    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGGGAGCAGATGGACCCTAC
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207091-plasmid-map-sequence-id-427374

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  9. px330-GAPDH

    Plasmid
    #136940
    Purpose
    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GAPDH (GAPDH Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136940-plasmid-map-sequence-id-272092

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  10. px330-CD4

    Plasmid
    #136938
    Purpose
    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting CD4 (CD4 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136938-plasmid-map-sequence-id-272090

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  18. pAAV-CMV-dSa VP64 Afp

    Plasmid
    #99697
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    99697-plasmid-map-sequence-id-194575

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  19. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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Showing: 11501 - 11520 of 14359 results