168,010 results

1. ML14

   Bacterial Strain

   #61911

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. tyrA gene deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMSCVpuro-Cas9-EGFP

   Plasmid

   #159126

   Purpose

   Expresses active Cas9 fused with EGFP for fluorescence microscopy

   Depositor

   Taekjip Ha

   Article

   Liu et al Science. 2020 Jun 12;368(6496):1265-1269

   Insert

   Cas9 fused to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MSCV LTR promoter

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ10704

   Plasmid

   #183957

   Purpose

   U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   SKSKSO array
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-TagBFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUW-VenusFlag-hCD44

   Plasmid

   #211824

   Purpose

   Express CD44 in mammalian cells

   Depositor

   Haian Fu

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   N-Terminal Venus and Flag tags

   Expression

   Mammalian

   Mutation

   isoform corresponds to UniProt ID P16070-1 with a…

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE2 mCherry-Rtn4a

   Plasmid

   #86683

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4a (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

   Plasmid

   #191172

   Purpose

   Human ADAR1, p150 isoform in plant-expressable format.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)
   

   Tags

   3FLAG and HIS

   Expression

   Plant

   Mutation

   WT

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRVV210

   Plasmid

   #87628

   Purpose

   Drives Bxb1 expression in the D. melanogaster germ line.

   Depositor

   Richard Mann

   Article

   Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

   Insert

   Bxb1
   

   Expression

   Insect

   Promoter

   vasa

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

   Plasmid

   #205550

   Purpose

   Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 SIRT7_H187Y

   Plasmid

   #53151

   Purpose

   expression vector for catalytically inactive human Sirtuin7

   Depositor

   Katrin Chua

   Article

   Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

   Insert

   Sirt7 (SIRT7 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H187Y, catalytic inactive

   Promoter

   CMV

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HIV-dual-GT

    Plasmid

    #122696

    Purpose

    Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

    Depositor

    Klaus Überla

    Article

    Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

    Inserts

    mTagBFP2
    DsRed-max
    

    Use

    Lentiviral

    Tags

    PEST domain

    Promoter

    HIV-1 LTR

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pnCas9-PBE

    Plasmid

    #98164

    Purpose

    Expresses CRISPR base editor in protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit

    #1000000191

    Purpose

    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

    Depositor

    Arnold Driessen

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

15. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GPR62-Tango

    Plasmid

    #66367

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR62 (GPR62 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRK-flag-USP19

    Plasmid

    #78597

    Purpose

    Express flag-USP19 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 (USP19 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid

    #223505

    Purpose

    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

    Depositor

    James Frank

    Insert

    SNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
    

    Tags

    SNAP-tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Cre

    Plasmid

    #198281

    Purpose

    Expresses Cre in mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Cre
    

    Expression

    Mammalian

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPM47 (UPR-RFP CEN/ARS URA3)

    Plasmid

    #20132

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    UPR-RFP
    

    Expression

    Yeast

    Available Since

    Feb. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge