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hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

Pooled Library

#83976

Purpose

Human CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
SV40 1: pBSSVD2005

Plasmid

#21826

Depositor

David Ron

Insert

SV40 Large T (SV40gp6 simian virus)

Expression

Mammalian

Mutation

likely expresses aa109-708 of the large T antigen

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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Ef1a_Large T-antigen_Ires_Puro

Plasmid

#18922

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

Large T Antigen (SV40gp6 Simian virus 40)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Article

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Tags

mCherry

Expression

Mammalian

Promoter

pTRE Tet ON

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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mini-pHelper-1.0

Plasmid

#230933

Purpose

The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Type

Empty backbone

Use

AAV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSGNluc

Plasmid

#186705

Purpose

A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

Depositor

Baubak Bajoghli, Maria Leptin

Article

Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

Inserts

GFP
luciferase

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_eGFP_TIA1

Plasmid

#106094

Purpose

gene expression of EGFP-TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP8

Plasmid

#45912

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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