We narrowed to 2,587 results for: PGK

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plenti-UbcP-Myc-YWAA-pGK-HYG

Plasmid

#107377

Purpose

Lentiviral expression of myc-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IRF4-3xHA-pGK-PUR

Plasmid

#107386

Purpose

Lentiviral expression of IRF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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N114 nLV EF-1a-Gal4-VP16-PGK-Puro

Plasmid

#44014

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Tags

VP16/Activation/Domain

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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WT1-2A-eGFP PGK PuroR Donor Plasmid

Plasmid

#82333

Purpose

WT1 targeting donor

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Inserts

WT1 5' Arm (WT1 Human)
WT1 3' Arm (WT1 )

Use

CRISPR and TALEN

Tags

2A-eGFP

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro GFP-LC3B G120

Plasmid

#123241

Purpose

Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

PGK

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-BICRAL-FLAG-pgk-puro

Plasmid

#107732

Purpose

Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

Depositor

Emily Dykhuizen

Article

Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

Insert

BICRAL (BICRAL Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IKZF4-3xHA-pGK-PUR

Plasmid

#107384

Purpose

Lentiviral expression of IKZF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF4 (IKZF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

Plasmid

#216664

Purpose

Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

Depositor

Leonardo Morsut

Inserts

ETV2 (Etv2 Mouse)
BFP
HygromycinR

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

Plasmid

#216666

Purpose

Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance

Depositor

Leonardo Morsut

Inserts

MyoD (Myod1 Mouse)
miRFP703
PuroR

Use

Lentiviral

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HRE mutant-Luc pGK Hygro

Plasmid

#118707

Purpose

reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HREmutant-Luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-NeoR-LoxP

Plasmid

#227503

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mScarletI-NLS. PGK-puro

Plasmid

#136263

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI-NLS

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W

Plasmid

#200504

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), SMARCA2(46) (SMARCA2, SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)

Plasmid

#215009

Purpose

Express a Lysophagy reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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SOX9-T2A-NLS-tdTomato-PGK-Puro

Plasmid

#89993

Purpose

Donor template for generation of SOX9-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

Plasmid

#195503

Purpose

SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)

Use

CRISPR; Donor vector

Tags

T2A-H2B-mCitrine

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCLJ-F2P-LoxP-PGK-PuroR-LoxP

Plasmid

#227502

Purpose

Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Prdm8 promoter

Use

AAV and Cre/Lox

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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