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We narrowed to 3,270 results for: Streptococcus

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Showing: 1141 - 1160 of 3270 results

  2. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  3. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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  5. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid
    #160587
    Purpose
    Nickase D10A Cas9 for Nt and Ct fusion.
    Depositor
    Diego Orzaez
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160587-plasmid-map-sequence-id-322079

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  6. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid
    #141108
    Purpose
    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis
    Depositor
    Matthew Gibson
    Publication
    Hill et al Methods Mol Biol. 2022;2450:437-465. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nematostella codon optimized Cas9
    Use
    Unspecified
    Mutation
    All amino acids coded for by codons optimized for…
    Promoter
    T7
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    141108-plasmid-map-sequence-id-314929

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  7. PX389 Fluviicola taffensis Cas9

    Plasmid
    #68326
    Purpose
    Fluviicola taffensis Cas9
    Depositor
    Feng Zhang
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68326-plasmid-map-sequence-id-119542

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  8. PX388 Bacteroides coprophilus Cas9

    Plasmid
    #68325
    Purpose
    Bacteroides coprophilus Cas9
    Depositor
    Feng Zhang
    Insert
    BcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68325-plasmid-map-sequence-id-125196

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  9. PX378 Sutterella wadsworthensis Cas9

    Plasmid
    #68315
    Purpose
    Sutterella wadsworthensis Cas9
    Depositor
    Feng Zhang
    Insert
    SwCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68315-plasmid-map-sequence-id-119526

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  10. PX387 Mycoplasma mobile Cas9

    Plasmid
    #68324
    Purpose
    Mycoplasma mobile Cas9
    Depositor
    Feng Zhang
    Insert
    MmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68324-plasmid-map-sequence-id-119539

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  11. PX392 Azospirillum B510 Cas9

    Plasmid
    #68329
    Purpose
    Azospirillum B510 Cas9
    Depositor
    Feng Zhang
    Insert
    AzoCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68329-plasmid-map-sequence-id-119551

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  12. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Publication
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  13. eSpCas9(1.1)

    Plasmid
    #138555
    Purpose
    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    eSpCas9(1.1)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138555-plasmid-map-sequence-id-274761

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  15. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid
    #101204
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101204-plasmid-map-sequence-id-198050

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  16. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid
    #101210
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101210-plasmid-map-sequence-id-198133

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  17. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid
    #101212
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    101212-plasmid-map-sequence-id-197040

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  18. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  19. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Publication
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110629-plasmid-map-sequence-id-210665

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Showing: 1141 - 1160 of 3270 results