We narrowed to 9,426 results for: ef1

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pHAGE-EF1aL-W308Rmut_ABCA3-GFP

Plasmid

#188550

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3 (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid W308->R308 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-EF1a-pA (JDW 1222)

Plasmid

#224526

Purpose

A Gateway compatible 3' entry clone containing rat EF-1a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF-1a polyA

Use

Gateway cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-HOXB5-DD-hygro

Plasmid

#222582

Purpose

PiggyBac transposon plasmid for Shield1-inducible expression of BCL2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

EF1a Promoter

Use

Synthetic Biology

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_HD_160-223AA

Plasmid

#221489

Purpose

NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD (TET+CTD)

Plasmid

#214082

Purpose

Mammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

hP53DD (TP53 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-V5-APEX2 (in pLEX_307)

Plasmid

#214921

Purpose

constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

V5-APEX2

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-noTag (in pLEX_307)

Plasmid

#214919

Purpose

constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-NlaCas7-p65

Plasmid

#207394

Purpose

Expresses NlaCas7-p65 activator fusion in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-NlaCas7-p65-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC8-NLS

Plasmid

#207395

Purpose

Expresses AcrIC8 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC8-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

Plasmid

#209776

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

Nluc, sgSTK4i, mRuby2 (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

Plasmid

#204622

Purpose

Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

mRFP1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a no GFP)

Plasmid

#172339

Purpose

mnucTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)TetR

Plasmid

#172352

Purpose

Tet Repressor reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

TetR
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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