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pIM41

Plasmid

#193535

Purpose

pENTRY clone for NLR Integrated Domain ID#84

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#84

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM39

Plasmid

#193533

Purpose

pENTRY clone for NLR Integrated Domain ID#82

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#82

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM38

Plasmid

#193532

Purpose

pENTRY clone for NLR Integrated Domain ID#65

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#65

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM35

Plasmid

#193529

Purpose

pENTRY clone for NLR Integrated Domain ID#90

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#90

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM34

Plasmid

#193528

Purpose

pENTRY clone for NLR Integrated Domain ID#60

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#60

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM31

Plasmid

#193525

Purpose

pENTRY clone for NLR Integrated Domain ID#91

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#91

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIM30

Plasmid

#193524

Purpose

pENTRY clone for NLR Integrated Domain ID#66

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#66

Use

Pentry clone

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::PpsbA2*::B0032::CYP110D1

Plasmid

#186707

Purpose

CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Promoter

PpsbA2*

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1

Plasmid

#186709

Purpose

CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Promoter

Ptrc.x.tetO2

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-6D

Plasmid

#149387

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

6bp deleted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-5D

Plasmid

#149388

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

5bp deleted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+5D

Plasmid

#149390

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

5bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+10D

Plasmid

#149391

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

10bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Opt

Plasmid

#149395

Purpose

P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

IHFprox site is optimized at key positions needed…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA.HA.HA.Mm.RAP1

Plasmid

#184646

Purpose

Mammalian expression of mouse Rap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfTpo

Plasmid

#168198

Purpose

Express zebrafish thrombopoietin (Tpo), mRNA synthesis

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlgb

Plasmid

#140293

Purpose

Express soluble His-tagged zebrafish Kitlgb in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDZ584 pKAN 1x-mCherry

Plasmid

#73172

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Use

Unspecified

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-p85α-ΔiSH2-C

Plasmid

#39499

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-C (PIK3R1 Bovine)

Use

Eukaryotic

Mutation

N488I, ΔiSH2-C = deletion of AA494-582

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-p85α-ΔiSH2-N

Plasmid

#39502

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Mutation

ΔiSH2-N = deletion of AA478-513

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only