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pSCT112: pTarget(AjTn6022)_U50D50

Plasmid

#200862

Purpose

Target plasmid containing attachement site for AjTn6022.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_comM

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pNS3_Ptrc::lasR_Plas::mNG

Plasmid

#189573

Purpose

Fluorescent reporter for Las activation by 3OC12-HSL

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Inserts

Transcriptional activator lasR
Monomeric Neon Green

Use

Synthetic Biology

Expression

Bacterial

Promoter

las and trc

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti

Plasmid

#228613

Purpose

Mammalian expression of a single fluorescent protein and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*Y152*

Plasmid

#191112

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 & Y152 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

Plasmid

#228508

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I5CHO (#1)

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pZB578_pB1H1_T7-RNAP_LARP-I

Plasmid

#228507

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Expression

Bacterial

Promoter

ugpB

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM315 pACYC-His6-Rpn10[ΔUIM]

Plasmid

#226348

Purpose

Expression of yeast Rpn10 with the UIM deleted

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

rpn10 (RPN10 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

delta UIM

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2620 MTK3-TetR

Plasmid

#226670

Purpose

MTK3 part containing the coding sequence of TetR

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

TetR

Use

Synthetic Biology

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2621 MTK3-LacI

Plasmid

#226671

Purpose

MTK3 part containing the coding sequence of LacI

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

LacI

Use

Synthetic Biology

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBWB507

Plasmid

#209325

Purpose

p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM

Plasmid

#207650

Purpose

Overexpression of native L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5c-cdl

Plasmid

#207649

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Tags

6xHis

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-cdl

Plasmid

#207652

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-decR

Plasmid

#207653

Purpose

Overexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

decR

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP4

Plasmid

#209966

Purpose

Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P40)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP-EV

Plasmid

#209973

Purpose

Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

CDS (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC53

Plasmid

#209962

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

5' Connector (5C1CSN_Tcat+sai)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PGK Promoter (MTK 2) (pAN2822)

Plasmid

#194224

Purpose

Mammalian Toolkit part 2 encoding the PGK promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK Promoter

Use

Synthetic Biology

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a Promoter

Use

Synthetic Biology

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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