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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 91,628 results for: cloning

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Showing: 11621 - 11640 of 91628 results

  1. pSRKBB-empty

    Plasmid
    #59449
    Purpose
    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.
    Depositor
    Claudia Schmidt-dannert
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    modified lac
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59449-plasmid-map-sequence-id-92571

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  2. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  3. pCLX-PGK-hCDK4

    Plasmid
    #114314
    Purpose
    Constitutive expression of the CDK4 protein
    Depositor
    Patrick Salmon
    Insert
    CDK4 (CDK4 Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114314-plasmid-map-sequence-id-218864

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  4. HRE:GFP-pGK:Cre

    Plasmid
    #68552
    Purpose
    Lentivirus expressing hypoxia-responsive GFP reporter and Cre
    Depositor
    Tyler Jacks
    Inserts
    Green Fluorescent Protein
    Cre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    HRE and pGK
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    68552-plasmid-map-sequence-id-120714

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  5. mCherry-Rab5C-S35N

    Plasmid
    #110503
    Purpose
    Dominant Negative
    Depositor
    Bernardo Mainou
    Insert
    Rab5C-S35N (RAB5C Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    S35N
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110503-plasmid-map-sequence-id-210729

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  6. pCAG-N2cG

    Plasmid
    #100811
    Purpose
    express rabies virus CVS N2c strain glycoprotein gene (N2cG)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain glycoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    100811-plasmid-map-sequence-id-189508

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  7. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

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  8. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  9. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

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  10. RARS_pNIC-Bio3

    Plasmid
    #153055
    Purpose
    Expression construct for producing Avi tagged Arginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    RARS (RARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153055-plasmid-map-sequence-id-304475

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  11. pRetroQ-Cre-ERT2

    Plasmid
    #59701
    Purpose
    to make Cre-ERT2 expressing stable cell line by retrovirus
    Depositor
    Richard Youle
    Insert
    Cre-ERT2
    Use
    Retroviral
    Tags
    Estrogen receptor (ERT)
    Expression
    Mammalian
    Promoter
    cmvIE
    Available Since
    Sept. 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    59701-plasmid-map-sequence-id-93474

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  12. DH-BB(5'SIN;TE12ORF)

    Plasmid
    #72309
    Purpose
    Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus
    Depositor
    Anthony Zador
    Insert
    DH-BB(5'SIN;TE12ORF)
    Use
    Sindbis virus helper construct
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72309-plasmid-map-sequence-id-145921

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  13. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

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  14. PG1424PGA

    Plasmid
    #74653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PG1424PGA
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. TARS_pNIC-Bio3

    Plasmid
    #153057
    Purpose
    Expression construct for producing Avi tagged Threonyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    TARS (TARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153057-plasmid-map-sequence-id-304477

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  16. PA-mCherry1-C1

    Plasmid
    #54495
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54495-plasmid-map-sequence-id-218682

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  17. PA-mCherry1-N1

    Plasmid
    #54507
    Purpose
    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

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  20. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Expression
    Bacterial
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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Showing: 11621 - 11640 of 91628 results