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pJH2829

Plasmid

#199693

Purpose

Pcex-1 tomm-20::miniSOG UrSL mCherry unc-54 3' UTR C.elegans RIM neuron expression of miniSOG RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

miniSOG

Tags

RFP

Expression

Worm

Promoter

Pcex-1

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL635

Plasmid

#189829

Purpose

The T-DNA backbone of Cas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

ccdb

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL639

Plasmid

#189833

Purpose

The T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

Plasmid

#212809

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His6-E3(1)-CUbo-TwinStrep

Plasmid

#212712

Purpose

Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

6xHis and CUbo-TwinStrep

Expression

Bacterial

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

Plasmid

#212803

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

NUbo and mCherry-FLAG

Expression

Mammalian

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-TET-NUbo-E3(63)-VSV

Plasmid

#212753

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-TET-NUbo-E3(48)-VSV

Plasmid

#212744

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(48)-VSV

Plasmid

#212743

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

Plasmid

#212800

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Mutation

HOIP(C885A)

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)

Plasmid

#212814

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo(K63R)

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(63)-FLAG

Plasmid

#212808

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

FLAG and NUbo

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

Plasmid

#211905

Purpose

Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD19-scFv

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His7-NUbo-GFP

Plasmid

#212791

Purpose

Copper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His7-NUbo-GFP

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo-GFP

Plasmid

#212792

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo-GFP

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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