We narrowed to 16,279 results for: LEA
-
Plasmid#166786PurposePhosphite dehydrogenase for NAD(P)H regenerationDepositorInsertPhosphite dehydroenase
TagsHis6ExpressionBacterialMutationA196R, T201S, A328T, E352N, and C356D- please see…PromoterT7Available SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEmCherry2
Plasmid#118413PurposeRed fluorescent reporter for assessing CRISPR gRNA activityDepositorInsertmCherry
UseCRISPRExpressionMammalianAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_nucl
Plasmid#113931PurposePlasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRETDepositorInsertH-Luc_nucl
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GINKO1
Plasmid#113111PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
Tags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialPromoteraraBADAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a_SMOX
Plasmid#186345PurposeC-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cellsDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-BFLF1
Plasmid#162657PurposeExpresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertBFLF1
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-YU2gp160
Plasmid#100927PurposeMammalian expression plasmid for Env from the YU-2 HIV-1 isolateDepositorInsertHIV-1 (YU2) Env
TagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10
Plasmid#127129DepositorAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-hNCOA6:V5
Plasmid#63585PurposeA subunit of the Trithorax-related (Trr) methyltransferase complex.DepositorAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-NES
Plasmid#36206PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046749
Plasmid#145629PurposeBacterial Expression plasmid for SARS-CoV-2 nsp11DepositorInsertSARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330_SpCas9_USP7 Exon 3 gRNA
Plasmid#131257PurposepX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7DepositorInserthumanised S. pyogenes Cas9 nuclease
ExpressionMammalianAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-StagRFP-EKAR-EV
Plasmid#173019PurposeRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.DepositorInsertsmURFP-StagRFP-EKAR-EV
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
TagsHexahistidine tag with Thrombin cleavageExpressionBacterialPromoterT7Available SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRExpressionPlantAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-CAAX
Plasmid#87612PurposePlasma membrane-targeted Venus (YFP).DepositorInsertVenus-CAAX
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230058PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
Tags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianPromoterChicken beta actin (CAG)Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230059PurposeExpresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
Tags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianPromoterChicken beta actin (CAG)Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only