165,803 results

1. pENN239_CRISPRcharm_Kv1

   Plasmid

   #220838

   Purpose

   CRISPRcharm Kv1 expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm Kv1
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSVL(D3)

   Plasmid

   #29335

   Depositor

   John Taylor

   Article

   Kuo et al J Virol. 1989 May . 63(5):1945-50.

   Insert

   Trimer of the HDV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUCsRNAP

   Plasmid

   #182746

   Purpose

   Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Insert

   small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)

   Viral Prep

   #51083-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (#51083). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato plasmid DNA. Cre-dependent GCaMP6f calcium sensor and physically separate nuclear tdTomato, under control of the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting

   Promoter

   EF1a

   Tags

   nuclear dTomato (physically separate, not a fusion protein)

   Available Since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-HA-NLGN3 WT

   Plasmid

   #59318

   Purpose

   Expression plasmid of HA-tagged wildtype human Neuroligin 3

   Depositor

   Peter Scheiffele

   Article

   Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

   Insert

   NLGN3 (NLGN3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV-mEGFP(A206K)-paCaMKII(K42M)

   Plasmid

   #165440

   Purpose

   Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(K42M)
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   K42M: Kinase dead mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCherryGFP-CoV2-WT

   Plasmid

   #177618

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

    Viral Prep

    #68717-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#68717). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. CAG-driven, Cre dependent, GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Promoter

    CAG-FLEX

    Tags

    mRuby2 (Cre-dependent)

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only