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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai3

Plasmid

#16370

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai3 (ORAI3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pLVX-PERK(LD)-EGFP-HOTag3

Plasmid

#225678

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Tu-His8

Plasmid

#224370

Purpose

Express EF-Tu protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-Tu (tufB E. coli)

Tags

His8

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Butelase AY

Plasmid

#221847

Purpose

Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

Val237Ala, Thr238Tyr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pC.HA Vav2

Plasmid

#14554

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav2 (VAV2 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMpGWBs02

Plasmid

#222478

Purpose

Gateway vector for 35s promoter-driven expression in Marchantia polymorpha carrying a sulfadiazine selection marker.

Depositor

Philip Carella

Article

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4

Plasmid

#61619

Purpose

FRET-based biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-Ts-His6

Plasmid

#224371

Purpose

Express EF-Ts protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-Ts (tsf E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-EF-G-His8

Plasmid

#224369

Purpose

Express EF-G protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

EF-G (fusA E. coli)

Tags

His8

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-ThrRS

Plasmid

#224365

Purpose

Express ThrRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

ThrRS (thrS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-CKM

Plasmid

#224375

Purpose

Express CKM protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

CKM (LOC107051134 Chicken)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-NDK

Plasmid

#224376

Purpose

Express NDK protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

NDK (ndk E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB-LgBiT

Plasmid

#182650

Purpose

FRB-LgBiT

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-LgBiT fusion

Tags

LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEPCTαKN0005

Plasmid

#187569

Purpose

Transcriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoter

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Luciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos15)GFP

Plasmid

#89248

Purpose

[Edit] Expresses GFP with +15 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos15)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPAN3

Plasmid

#37374

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsPAN3 (PAN3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PAsfCherry2(1-10)

Plasmid

#82612

Purpose

Express PAsfCherry2(1-10) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

PAsfCherry2(1-10)

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-CD81-10

Plasmid

#57124

Purpose

Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

CD81 (CD81 Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits